Regulatory

Part:BBa_K112323:Design

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-22)


{H-NS!} in BBb format


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR ca998/JH090R on pBjh1601AC-K112321-2    (445 bp, EcoRI/BamHI)
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
Product is pBjh1601AC-K112323
-----------------------------------------------
ca998   Forward biobricking of {H-NS!} Salmonella   
gtatcacgaggcagaatttcag
JH090R   Reverse biobricking of {H-NS!} Salmonella   
cgttaGGATCCTTATTcCTTGATCAGG



Source

pBjh1601AC-K112321-2

References